Steps towards an Arthrobacter
nicotinovorans biotechnology for
- Research project financed by the Ministry of Education and Scientific Research - Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI), contract no. 175PED/28.08.2017
amount - 475000
lei (aprox. 100 000 euros);
- Funding period - 16 months (28.08.2017 - 31.12.2018).
6-hidroxy-nicotine (6HNic), a naturally occurring metabolite
of Arthrobacter nicotinovorans was shown to bind the
nicotinic acetylcholine receptors and to modulate their
function. Experimental tests using laboratory rats have
shown the ability of 6HNic to cope with the acetylcholine
depletion and to restore normal brain functions.
6HNic has been thereby identified as a high impact bio-pharmaceutical with application in the therapy of
neurodegenerative disorders associated with low acetylcholine levels.
The scope of the project is to develop a technology for the production of the neuroprotective agent 6HNic in an industrial-like environment and to test it at a pilot scale. For this, a genetically engineered Arthrobacter strain will be created by knocking-out specific genes using suicidal vectors. The mutant strain will be further accommodated to a bio-reactor and the conditions for optimum 6HNic production will be evaluated. The final goal is to formulate a microbial based biotechnology for the production of 6HNic ready to be transferred to the industrial environment.
||Project coordinator, Biochemistry, Molec. Biol.||More details...|
||Microbiology, Immunology||More details...|
||Neurophysiology, Behavior||More details...|
|PhDs and students|
||Biochemistry and Behavior|
||Molecular Biology, Biochemistry and Behavior|
Position on offer - details UAIC ANCS Jobs - position not available, occupied by Boiangiu Răzvan, PhD student
Project's objectives and activities:
- pdf file only in Romanian
List of publications supported by the project:
1. Mihasan, M.; Babii, C.; Aslebagh, R.; Channaveerappa, D.; Dupree, E.; Darie, C. C., Proteomics based analysis of the nicotine catabolism in Paenarthrobacter nicotinovorans pAO1, Scientific Reports, 2018, 8, Article number: 16239 [Fulltext]
Marius Mihăşan, PhD
Adress : Room B228, Biochemistry and Molecular Biology Laboratory, Faculty of Biology, Alexandru I Cuza University of Iasi, Carol I B-vd., No. 20A, Iaşi, 700506, Romania