genome of a useful bacteria: Paenarthrobacter
nicotinovorans – next step in extending it’s
PN-III-P4-ID-PCE-2020-0656, No. PCE 152 / 2021
project funded by the Ministry of Education and
Scientific Research - Executive Unit for Financing
Higher Education, Research, Development and Innovation
(UEFISCDI), PNCDI III - P4 - Fundamental and frontier
amount - 1.198.032 lei (approx. 246.000 euro);
- Funding period - 36 months (4.01.2021 - 32.12.2023).
Paenarthrobacter nicotinovorans pAO1 is a nicotine degrading microorganism that shows promising applications in converting nicotine-containing waste into useful green chemicals. Its biotechnological applications are nevertheless hampered by the lack of knowledge and tools to perform genetic and metabolic engineering. After solving the nicotine-induced proteome of this bacteria, the project leader of the current proposal concluded that none of the draft genomes available for this strain are complete. The current application aims to provide the first complete and annotated genome of P. nicotinovorans pAO1, and, as such, the first complete genome of a nicotine degrading microorganism. In order to achieve this, P. nicotinovorans pAO1 gDNA will be isolated and whole-genome sequencing (WGS) will be performed using two platforms: NGS Illumina for reading depth and ONT MinION for scaffolding and assembling the genome. The quality of the assembly and annotation will be addressed using dRNA-seq and the already available raw proteomics data. The project will not only strengthen the much need bioinformatics know-how in the already existing team of the project leader, but also will group allow the group to generate a genome-scale metabolic model of the nicotine metabolism, and, consecutively, to develop high yield bio-technologies for production on nicotine derivatives..
|Alexandru Ioan Cuza
University of Iași
||Project coordinator||More details|
||Biochemistry and Molecular Biology||More
|PhDs and students|
||starting on 1 October 2022||Details
|MunteanuIustin Tiberius||masters student|
July 11th, 2022 - An overview of our activity at FEBS IUBMB-FEBS-PABMB 2022
July 11th, 2022 - Team member Tiberius Iustin Munteanu presenting his work at IUBMB-FEBS-PABMB2022 - The Biochemistry Global Summit, Lisbon, Portugal.
July 2nd, 2022 - Presenting the posters at FEMS 2022, Belgrade, Serbia
June 28th, 2022 - Poster on P.nicotinovorans nicotine metabolism presented at FEMS 2022, Belgrade, Serbia
June 28th, 2022 - Poster on P. nicotinovorans genome presented at FEMS 2022, Belgrade, Serbia
June 10th, 2022 - Poster on nicotine metabolism presented at ASMS 2022, Minneapollis, MN, USA with Darie Proteomics & Biochemistry Group.
June 2nd, 2022 - Marius Mihasan, PI, talking about the results of the project at Clarkson University, Potsdam, NY, USA
May 30, 2022 - Marius Mihasan, PI, talking about the results of the project at Marmara University, Istanbul, Turkey
April 25th, 2022 - New manuscript accepted for publication
Marius Mihășan, PhD
Phone: 40(0)232201102 - 1524
Further details: here