Sequencing the genome of a useful bacteria: Paenarthrobacter nicotinovorans – next step in extending it’s biotechnological applications
PN-III-P4-ID-PCE-2020-0656, No. PCE 152 / 2021

  • Research project funded by the Ministry of Education and Scientific Research - Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI), PNCDI III - P4 - Fundamental and frontier research
  • Funding amount  - 1.198.032 lei (approx. 246.000 euro);
  • Funding period - 36 months (4.01.2021 - 32.12.2023).

Project summary:

    Paenarthrobacter nicotinovorans pAO1 is a nicotine degrading microorganism that shows promising applications in converting nicotine-containing waste into useful green chemicals. Its biotechnological applications are nevertheless hampered by the lack of knowledge and tools to perform genetic and metabolic engineering. After solving the nicotine-induced proteome of this bacteria, the project leader of the current proposal concluded that none of the draft genomes available for this strain are complete. The current application aims to provide the first complete and annotated genome of P. nicotinovorans pAO1, and, as such, the first complete genome of a nicotine degrading microorganism. In order to achieve this, P. nicotinovorans pAO1 gDNA will be isolated and whole-genome sequencing (WGS) will be performed using two platforms: NGS Illumina for reading depth and ONT MinION for scaffolding and assembling the genome. The quality of the assembly and annotation will be addressed using dRNA-seq and the already available raw proteomics data. The project will not only strengthen the much need bioinformatics know-how in the already existing team of the project leader, but also will group allow the group to generate a genome-scale metabolic model of the nicotine metabolism, and, consecutively, to develop high yield bio-technologies for production on nicotine derivatives..

Project members: 

Alexandru Ioan Cuza University of Iași

  • Marius Mihășan, PhD 
Project coordinator
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  • Marius Ştefan, PhD
  • Răzvan Ștefan Boiangiu
Biochemistry and Molecular Biology
More details
  • Amada El-Sabeh

PhDs and students

  • One PhD position still available
starting on 1 October 2022
Details here
  • Students

Honceriu Iasmina
master student

MunteanuIustin Tiberius masters student

      News       Objectives and progress reports    Publications 

October 25, 2022 - Members of the project team attended the SRBC 2022 Meeting, Cluj-Napoca, Romania

October 12, 2022 - Our Paenarthrobacter nicotinovorans ATCC 49919 genome was included in the Prokaryotic Representative Genomes  Collection

September14, 2022 - lab life for a bioinformatician assemblying bacterial genomes

August 30, 2022 - Boiangiu Razvan Stefan presenting his work at #PSEMeeting2022

August 25, 2022 -First successful experiment for directly sequencing bacterial RNA

August 23, 2022 - New article published by the youngest member of the team -Munteanu Iustin Tiberius

July 11th, 2022 - An overview of our activity at FEBS IUBMB-FEBS-PABMB 2022

July 11th, 2022 - Team member  Amada El-Sabeh presenting her work at IUBMB-FEBS-PABMB2022 - The Biochemistry Global Summit, Lisbon, Portugal. 

July 11th, 2022 - Team member  Tiberius Iustin Munteanu presenting his work at IUBMB-FEBS-PABMB2022 - The Biochemistry Global Summit, Lisbon, Portugal.

July 2nd, 2022 - Presenting the posters at FEMS 2022, Belgrade, Serbia

June 28th, 2022 - Poster on P.nicotinovorans nicotine metabolism presented at FEMS 2022, Belgrade, Serbia

June 28th, 2022 - Poster on P. nicotinovorans genome presented at FEMS 2022, Belgrade, Serbia

June 10th, 2022 - Poster on nicotine metabolism presented at ASMS 2022, Minneapollis, MN, USA with  Darie Proteomics & Biochemistry Group.

June 2nd, 2022 - Marius Mihasan, PI, talking about the results of the project at Clarkson University, Potsdam, NY, USA

May 30, 2022 -  Marius Mihasan, PI, talking about the results of the project at Marmara University, Istanbul, Turkey

May 10th, 2022 -  Paper announcement paper is finally available and genome released to the public

April 25th, 2022 - New manuscript accepted for publication

April 12, 2022 - Marius Mihasan talking about bacterial genome sequencing at Clarkson University, Potsdam, NY, USA

November 28th, 2021 - Tiberius Munteanu presenting his work at YSF 2022

August 9th, 2021 - New goodies in the lab!

July 22, 2021 - Poster presented at Young Researchers in BioSciences 2021:

June 29, 2021 - Poster presented at FEBS 2021:

June 25, 2021 - Poster presented at FEBS 2021:

June 16th, 2021 - A new dataset released on Pride database

October 7th, 2021 - Protein nanopores give green light for DNA sequencing for the first time in our lab

June 8th, 2021 - New goodies for the lab:
May 26, 2021 - New publication on nicotine metabolism:


Project manager:

Marius Mihășan, PhD

Adress: Room B228, Biochemistry and Molecular Biology Laboratory, Faculty of Biology, Alexandru I Cuza University of Iasi, Carol I B-vd., No. 20A, Iaşi, 700506, Romania
Phone:   40(0)232201102 - 1524
Fax:         40(0)232201472
Further details: here
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