Cercetare


Tema

21/01/2020

Microorganismul Paenarthrobacter nicotinovorans (numit anterior Arthrobacter nicotinovorans) este unul din putinele organisme vii ce are capaciåtatea de a descompune alcaloidul nicotina si de a-l utiliza ca sursa de carbon si  nitrogen. Proprietate se datoreaza prezentei mega-plasmidului pAO1, entitate genetica independenta avand dimensiunea de 165 kB. pAO1 a fost complet secventiat si contine 2 grupe mari de gene: un prim grup implicat in metabolismul nicotinei si al doilea grup ce formeaza un operon implicat in metabolismul glucidelor. Este acceptat faptul ca genele implicate in catabolismul nicotinei au fost recrutate prin transfer orizontal de gene si grefate pe un plasmid ancestral, ce continea operonul implicat in metabolismul glucidelor (Igloi &  Brandsch 2003).



Mega-plasmidul pAO1 din Paenarthrobacter nicotinovorans (cu albastru - gene implicate in metabolismul nicotinei, verde - gene implicate in metabolismul glucidelor, rosu - gene implicate in procesele de recombinare, alb - gene cu functie necunoscuta)
















Principala tema de cercetare este organizarea moleculara si biologia megaplasmidul pAO1, in special in legatura cu mecanismele de protectie impotriva stresului oxidativ generat de metabolizarea nicotinei, metabolismul  glucidelor si aplicatiile biotehnologice ale acestor 2 cai. Utilizand o combinatie de tehnici bioinformatice am identificat substrate posibile pentru calea metabolica de degradare a glucidelor codificate de pAO1. Compusii selectati au fost apoi testati si am identificat ca substratul metabolic al celui de-al doilea grup de gene de pe pAO1 este xiloza.


Operonul codificand calea de degradare oxidativa a xilozei codificate de pAO1.


In ultimii ani prioritara a devenit evaluarea aplicatiilor medicale ale derivatilor nicotinici produsi de Paenarthrobacter nicotinovorans. Utilizand metode bioinformatice am testat potentialul derivatilor nicotinici de a se de receptorii nicotinici ai acetilcolinei. Am identificat astfel 6-Hydroxy-Nicotine (6HNic)  ca un potential ligand al receptorilor mentionati. In collaborare cu  dr. Lucian Hritcu am demonstrat ca intradevar 6HNic poate modula activitatea receptorilor nicotinici si imbunatati memoria intr-un model animal al bolii Alzheimer. In prezent, dezvoltam o metoda biotehnologica de producere a 6HNIC.


Cultura de Paenarthrobacter nicotinovorans intr-un microfermentator de 3L.



O cultura de 48 ore vizualizata in 1 min si 30 sec, credit: Lector. Dr. Eugen Ungureanu.

    Proiectele recente au ca scop studil integrat genomic, transcriptomic si proteomic al tulpinii Paenarthrobacter nicotinovorans pAO1 și a căii de degradare a nicotinei existente în aceast microorganism.

Pana acum, laboratorul coordonat a produs si facut publice urmatoarele seturi de date relevante:

  • primul genom complet si adnotat al microorganismului Paenarthrobacter nicotinovorans ATCC 49199: GenBank CP089293 si CP089294, precum si datele brute asociate PRJNA693273

  • un set de date de transcriptomica ce descriu metabolismul nicotinei in Paenarthrobacter nicotinovorans ATCC 49199: Gene Expression Omnibus GSE240220

  • un set de date de proteomica ce identifica proteinele exprimate in Paenarthrobacter nicotinovorans in prezenta nicotinei folosind nanoLCMSMS PXD008756

  • un set de date de proteomica ce descrie dinamica expresiei enzimelor implicate in metabolizarea nicotinei in Paenarthrobacter nicotinovorans - PXD012577

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Finantare

04/04/2021

  • PN-III-P4-ID-PCE-2020-0656, Sequencing the genome of a useful bacteria: Paenarthrobacter nicotinovorans – next step in extending it’s biotechnological applications

  • PN-III-P2-2.1-TE-2016-0367 - Developing an Arthrobacter nicotinovorans biotechnology for neuro-pharmaceuticals production

  • PN-III-P2-2.1-PED-2016-0177 - Steps towards an Arthrobacter nicotinovorans biotechnology for neuro-pharmaceuticals production

  • PN-II 50BM/2016 - Romania - P.R.P. China joint research project - Nicotine - from toxic residue to metabolic derivatives with neuroprotective effects

  • PN-II-RU-TE-2014-4-0106 - Effects of 6-hydroxy-nicotine on chlorisondamine-induced oxidative stress and neurotoxicity: relevance for Alzheimer’s disease

  • Proiectul de cercetare GI-2014-02 - Grant de cercetare intern pentru tineri cercetători acordat de către Universitatea “Alexandru Ioan Cuza” din Iaşi

  • Proiectul de cercetare CNCSIS TD-236/2007, Implicatiile moleculare ale megaplasmidului pAO1 in metabolismul speciei Arthrobacter nicotinovorans

  • Proiectul de cercetare UEFISCDI PD-337/2010, Clonarea si caracterizarea cadrelor de lectura ORF32 and ORF40 de pe megaplasmidul pAO1 - potentiale modele de studiu a interactiunilor tagatoza-proteine

Publicatii

20/12/2024

Articole în ultimii 5 ani
  1. https://pmc.ncbi.nlm.nih.gov/articles/PMC11643659/Boiangiu, R.S.; Brinza, I.; Honceriu, I.; Mihasan, M.; Hritcu, L. Insights into Pharmacological Activities of Nicotine and 6-Hydroxy-L-nicotine, a Bacterial Nicotine Derivative: A Systematic Review. Biomolecules 2024, 14, 23.  [Free FullText]
  2. Brinza, I., Boiangiu, R. S., Mihasan, M., Gorgan, D. L., Stache, A. B., Abd-Alkhalek, A., El-Nashar, H., Ayoub, I., Mostafa, N., Eldahshan, O., Singab, A. N., & Hritcu, L. (2024). Rhoifolin, baicalein 5,6-dimethyl ether and agathisflavone prevent amnesia induced in scopolamine zebrafish (Danio rerio) model by increasing the mRNA expression of bdnf, npy, egr-1, nfr2α, and creb1 genes. European Journal of Pharmacology, 984, 177013 [Free Fulltext]
  3. El-Sabeh, A., Mlesnita, AM., Munteanu, IT. .... Mihasan M. Characterisation of the Paenarthrobacter nicotinovorans ATCC 49919 genome and identification of several strains harbouring a highly syntenic nic-genes cluster. BMC Genomics 24, 536 (2023). [Free Fulltext]
    Pérez-Ramos, A.; Ladjouzi, R.; Mihasan, M.; Teiar, R.; Benachour, A.; Drider, D. Advances in Characterizing the Transport Systems of and Resistance to EntDD14, A Leaderless Two-Peptide Bacteriocin with Potent Inhibitory Activity. Int. J. Mol. Sci. 2023, 24, 1517. [Free Fulltext]
  4. Amada El-Sabeh, Iasmina Honceriu, Fakhri Kallabi, Razvan-Stefan Boiangiu, Marius Mihasan - Complete Genome Sequences of Two Closely Related Paenarthrobacter nicotinovorans Strains. Microbiol Resour Announc. 2022; 11(6):e0013322. doi: 10.1128/mra.00133-22. [Fulltext]
  5. Madi-Moussa, D.; Deracinois, B.; Teiar, R.; Li, Y.; Mihasan M.; Flahaut, C.; Rebuffat, S.; Coucheney, F.; Drider, D. Structure of Lacticaseicin 30 and Its Engineered Variants Revealed an Interplay between the N-Terminal and C-Terminal Regions in the Activity against Gram-Negative Bacteria. Pharmaceutics 2022, 14, 1921. doi:10.3390/pharmaceutics14091921 [Fulltext]
  6. Postu, P.A.; Mihasan, M.; Gorgan, D.L.; Sadiki, F.Z.; El Idrissi, M.; Hritcu, L. Pinus halepensis Essential Oil Ameliorates Aβ1-42-Induced Brain Injury by Diminishing Anxiety, Oxidative Stress, and Neuroinflammation in Rats. Biomedicines 2022, 10, 2300. doi:10.3390/biomedicines10092300 [Fulltext]
  7. Mihasan M., Boiangiu R.S., Guzun D., Babii C., Aslebagh R., Channaveerappa D., Dupree D., Darie CC. Time-dependent analysis of Paenarthrobacter nicotinovorans pAO1 nicotine-related proteome. ACS Omega 2021, 6, 22, 14242–14251 [Fulltext]
  8. Mihasan, M. A Beginner’s Guideline for Low‐cost 3D Printing of Macromolecules Usable for Teaching and Demonstration. Biochem. Mol. Biol. Educ. 2021, bmb.21493. [Fulltext]
  9. Postu, P. A.; Tiron, A.; Tiron, C. E.; Gorgan, D. L.; Mihasan, M.; Hritcu, L. Conifer Essential Oils Reversed Amyloid Beta1-42 Action by Modulating BDNF and ARC Expression in The Rat Hippocampus. CNS Neurol. Disord. - Drug Targets 2021, 20. [Fulltext]
  10. Boiangiu, R. S.; Mihasan, M.; Gorgan, D. L.; Stache, B. A.; Hritcu, L. Anxiolytic, Promnesic, Anti-Acetylcholinesterase and Antioxidant Effects of Cotinine and 6-Hydroxy-L-Nicotine in Scopolamine-Induced Zebrafish (Danio Rerio) Model of Alzheimer’s Disease. Antioxidants 2021, 10 (2), 212. [Free Fulltext]
  11. Brandsch, R and Mihasan M, A soil bacterial catabolic pathway on the move: Transfer of nicotine catabolic genes between Arthrobacter genus megaplasmids and invasion by mobile elements, Journal of Biosciences, 2020, 45, 1-12  [Fulltext]
  12. Djeussi, D. E.; Noumedem, J. A. K.; Mihasan, M.; Kuiate, J. R.; Kuete, V. Antioxidant Activities of Methanol Extracts of Thirteen Cameroonian Antibacterial Dietary Plants. J. Chem. 2020, 2020. [Free Fulltext]
  13. Dupree EJ, Jayathirtha M, Yorkey H, Mihasan M, Petre BA, Darie CC, A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of this Field, Proteomes, 2020, 8 (3), 14 [Fulltext]
  14. Boiangiu RS, Mihasan M, Gorgan DL, Stache BA, Petre BA, Hritcu L., Cotinine and 6-Hydroxy-L-Nicotine Reverses Memory Deficits and Reduces Oxidative Stress in Aβ25-35-Induced Rat Model of Alzheimer’s Disease, Antioxidants, 2020, 9(8), 768 [Fulltext]

Lista completă de publicații este disponibilă aici

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25/10/2024

Postere/Prezentari în ultimii 5 ani


  1.  Marius Mihasan A Guideline for 3D Printing Macromolecular Models on the Cheap, Molecular Life Sciences Education and Training Conference, March 20-23, 2024, Antalya, Turcia [Photo] [Photo] [Poster]
  2. Mihășan Marius; Boiangiu Răzvan-Ștefan; Popa Laura Nicoleta, Impact Of 3D-printed molecular models on teaching protein and DNA structure, Natural Sciences in the Dialog of Generations VIIth Edition, Universitatea de Stat din Moldova, Chișinău, Rep. Moldova, 12-13.09.2024 [Photo] [Photo][Presentation]
  3. R.S. Boiangiu, M. Mihasan, Impact of 3D-printed molecular models on teaching – a compensatory research study, FEBS48th Congress: Mining biochemistry  for human health and well-being. Milano, Italia, 28.06.2024-03.07.2024 [Photo] [Poster]
  4. Marius Mihășan, Putting molecules from the computer screen into students’ hands using 3D printing.  Education work-shop Promoting visual literacy in bio-molecular sciences education, Bucharest, 24 September 2024; [Photo] [Photo] [Presentation]
  5. El-Sabeh, A., Mlesnita, AM., Munteanu, IT. .... Mihasan M., A highly syntenic nic-genes cluster is present in several related bacterial strains, Conferinta Societatii Nationale de Biochimie si Biologie Moleculara, 12-15 Septembrie, Cluj, Romania, [Photo] [Poster].
  6. Marius Mihășan, Abdelrahman Mohamed Taha Mahmoud, Putting figures from Lehninger’s textbook on the table using 3D printing47th FEBS Congress – ‘Together in bioscience for a better future’, Tours, France,  8th - 12th July 2023,  [Poster].
  7. El-Sabeh, A., Mlesnita, AM., Munteanu, IT. .... Mihasan M., A highly syntenic nic-genes cluster is present in several related bacterial strains, 47th FEBS Congress – ‘Together in bioscience for a better future’, Tours, France,  8th - 12th July 2023, [Photo] [Poster].
  8. Mihasan Marius - Full omics caracterisation of a bacteria. Case study - Paenarthrobacter nicotinovorans pAO1 and its nicotine metabolism, Salerno University, Host Assoc. Prof. Anna Maraboti, May 26, 2023 [Presentation]
  9. Mihasan Marius - 3D printed macromolecular models usable for teaching and demonstration, Salerno University, Host Assoc. Prof. Anna Maraboti, May 26, 2023 [Photo] [Photo] [Presentation]
  10. Mihasan Marius - Multi-omics characterisation of nicotine metabolism in Paenarthrobacter nicotinovorans, The 2023 RoBioinfo Conference, 11th-13th May 2023,  [Photo] [Photo] [Presentation]
  11. Mihasan Marius - Steps towards the multi-omics characterization of nicotine metabolism in Paenarthrobacter nicotinovorans ATC 49919, Smart Diaspora 2023, Timisoara, 10th-13th April 2023 [Presentation]
  12. Mihasan Marius - Lessons learned from 3D printing macromolecular models for teaching and demonstration-  The IUBMB-FEBS-PABMB 2022 Congress - The Global Biochemistry Summit, 9-14 July 2022, Lisabona, Portugal, Best Education section poster award by SPB [Photo] [Photo] [Photo] [Photo] [Poster]
  13. I.T. Munteanu, A.M. Mleșnița, F. Kallabi, M. Mihașan - In silico evaluation of antibiotic resistance andCRISPR systems of Paenarthrobater nicotinovorans - The IUBMB-FEBS-PABMB 2022 Congress - The Global Biochemistry Summit, 9-14 July 2022, Lisabona, Portugal*
  14. A. El-Sabeh, I. Honceriu, F. Kallabi, R. Boiangiu, M. Mihașan - Genome sequence of Paenarthrobacter nicotinovorans strain ATCC 49919, a nicotine- degrading soil microorganism, The IUBMB-FEBS-PABMB 2022 Congress - The Global Biochemistry Summit, 9-14 July 2022, Lisabona, Portugal*
  15. Amada El-Sabeh, I. Honceriu, F. Kallabi, R. Boiangiu, M. Mihășan. - Complete genome sequence of the well-studied nicotine-degrading microorganism Paenarthrobacter nicotinovorans strain ATCC 49919, FEMS Conference on Microbiology, June 29 - July 2nd, 2022, Belgrade, Serbia [Photo] [Poster]
  16. Mihasan M., Boiangiu R.S., Guzun D., Babii C., Aslebagh R., Channaveerappa D., Dupree D., Darie CC. - Proteome-wide analysis of Paenarthrobacter nicotinovorans ATCC 49919 nicotine- metabolism, FEMS Conference on Microbiology, June 29 - July 2nd, 2022, Belgrade, Serbia [Photo] [Poster]
  17. Mihasan M., Boiangiu R.S., Guzun D., Babii C., Aslebagh R., Channaveerappa D., Dupree D., Darie CC., Integration and regulation of nicotine metabolism in Paenarthrobacter nicotinovorans as revealed by proteomic data.
    70th ASMS Conference on Mass Spectrometry and Allied Topics, June 5-9 2022, Minneapolis, Minnesota, USA [Photo] [Photo] [Poster]
  18. Mihasan, M. 3D printed macromolecular models usable for teaching and demonstration Marmara University, Host Dr. Beste Turanli June 2022, Istanbul, Turkey [Photo] [Photo]
  19. Mihasan, M. Full omics characterization of a bacteria.  Case study - Paenarthrobacter nicotinovorans pAO1 and its nicotine metabolism,  Marmara University, Host Dr. Beste Turanli June 2022, Istanbul, Turkey [Photo] [Photo]
  20. Mihasan, M. Full omics characterization of a bacteria.  Case study - Paenarthrobacter nicotinovorans pAO1 and its nicotine metabolism,  Clarkson University, Host Dr. Costel C. Darie, June 2022, Potsdam, NY, USA, [Flyer] [Photo] [Presentation]
  21. Mihasan, M., Genome sequencing as a tool to unlock the full biotechnological potential of bacteria,  Clarkson University, School of Arts & Sciences, Walsh Seminar Series, Host Dr. Costel C. Darie, April 2022, Potsdam, NY, USA, Virtual [Flyer] [Presentation]
  22. Mihasan, M. 3D printed macromolecular models usable for teaching and demonstration, November 2020, Lille, France  [Photo] [Photo] [Presentation]
  23. Mihasan, M. A guideline for 3D printing of macromolecular models on the cheap, EMBL Conference: Bringing Molecular Structure to Life: 50 Years of the PDB, 20 - 22 October 2021, Virtual, [Poster]
  24. Mihasan M  - Using molecular models for a better education in Life Sciences, October 2021, Iasi, Romania, [Photo] [Photo] [Presentation]
  25. Mihasan Marius, F. Kallabi - Draft genome of a useful bacteria - Paenarthrobacter nicotinovorans, The 45th FEBS Congress - Molecules of Life: Towards new horizons, 2-8 July, 2021, Virtual,  [Poster]
  26. Mihasan, M. A guideline for 3D Printing of macromolecular models on the cheap, PDB50: A special symposium celebrating the 50th anniversary of the Protein Data Bank, May 4, 2021 - May 5, 2021, Virtual [Poster] [Poster presentation]
  27. Mihasan, M., 3D printed macromolecular models usable for teaching and demonstration,  Clarkson University, School of Arts & Sciences, Walsh Seminar Series, Host Dr. Costel C. Darie, April 2021, Potsdam, NY, USA, Virtual [Flyer] [Presentation]
  28. Mihasan, M., 3D printed macromolecular models usable for teaching and demonstration? Clarkson University, School of Arts & Sciences, Walsh Seminar Series, Host Dr. Costel C. Darie, April 2021, Potsdam, NY, USA, Virtual [Flyer] [Presentation]
  29. Mihășan M., Brandsch R., Steps towards revealing the evolution of nicotine catabolic gene clusters in Arthrobacters, EMBO conference Molecular Mechanisms in Evolution and Ecology, 30 September - 2nd October 2020, Virtual, [Poster]
  30. Mihăşan M, Babii C, Aslebagh R, Channaveerappa D, Dupree EJ, Darie CC. Proteomic Analysis of Nicotine Metabolism in Paenarthrobacter Nicotinovorans, Eastern Analytical Symposium, November 16-19, 2020 [Presentation Abstract][Recorded presentation]
  31. Darie CC, Dupree EJ, Jayathirtha M, Yorkey H, Mihasan M, Petre BA, Proteomics: The Good, the Bad, and the Future of this Field, Eastern Analytical Symposium, November 16-19, 2020 [Presentation Abstract]

Carti și capitole de carte:


  1. Mihăşan, M., Babii, C., Aslebagh, R., Channaveerappa, D., Dupree, E.J., Darie, C.C., 2019. Exploration of Nicotine Metabolism in Paenarthrobacter nicotinovorans pAO1 by Microbial Proteomics, in: Woods, A.G., Darie, C.C. (Eds.), Advancements of Mass Spectrometry in Biomedical Research, Vol. 1140 of Advances in Experimental Medicine and Biology. pp. 515–529.  [Fulltext]

  2. Mihăşan, M., Wormwood, K.L., Sokolowska, I., Roy, U., Woods, A.G., Darie, C.C., 2019. Mass Spectrometry- and Computational Structural Biology-Based Investigation of Proteins and Peptides, in: Woods, A.G., Darie, C.C. (Eds.), Advancements of Mass Spectrometry in Biomedical Research, Vol. 1140 of Advances in Experimental Medicine and Biology. pp. 265–287.  [Fulltext]

  3. Hritcu, L., Mihasan M., 2019. 6-Hydroxy-l-Nicotine and Memory Impairment 6-Hydroxy-l-Nicotine and Memory Impairment, book chapter in Neuroscience of Nicotine Mechanisms and Treatment, 1st Edition, Editor Preedy V, pages 165-172 [Fulltext]

  4. Mihasan Marius -  Megaplasmidul pAO1 - structura si functie, Editura Universitatii ''Alexandru Ioan Cuza'' din Iasi, 2011, carte finantata de catre CNCSIS-UEFISCSU, PN II-RU 337/2010 [carte in format .pdf]

  5. Marius Mihasan, Marius Stefan, Zenovia Olteanu -  Biologie moleculară - metode experimentale, Editura Universitatii ''Alexandru Ioan Cuza'' din Iasi, 2012, carte finantata de catre CNCSIS-UEFISCSU, PN II-RU 337/2010 [.pdf]

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Colaborari

20/07/2017

  • Grupul de Biochimie si Proteomica condus de Dr. Costel C. Darie, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, USA - a facut posibila realizarea unui stagiu de cercetare finantat de Comisia Fulbright Romania. Dr. Darie a pus la dispozitie reactivi, laboratorul si instrumente pentru a stabili proteomul bacteriei Arthrobacter nicotinovorans.


Grupul dr. Darie, Aprilie 2017. De la dreapta la stanga Dr. Darie, Kelly W., Roshi A., Devika C., Emma D. si eu in tricolul cu porcul.


  • Dr. Hongzhi Tang (Robert) şi  Dr. Fei Tao, Group for Food & Environmental Micro-Biotechnol, State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University

In vizita la School of Life Sciences & Biotechnology, Shanghai Jiao Tong University.
De la dreapta la stanga Dr. Fei Tao, Hritcu L., Olteanu Z., Stefan M, eu si Dr. Hongzhi Tang.

  • Grupul condus de prof. dr. Roderich Brandsch, Institut für Biochemie und Molekularbiologie este cel in care mega-plasmidul pAO1 a fost secventiat, asa ca ei sunt cei ne care ofera informatii importante  legate de biologia moleculara a acestui mega-plasmid.


Prof. dr. Roderich Brandsch explicand conceptul sau in arta, Septembbrie 2010, Freiburg (de la stanga la dreapta: dr. Marius Mihasan, prof. dr. Roderich Brandsch, dr. Eugen Ungureanu)

  • Toate problemele legate de enzime si cinetica enzimatica sunt discutate cu prof. dr. Vlad Artenie, Universitatea ''Alexandru I Cuza'', Facultatea de Biologie

  • Detaliile practice legate de operatii ce implica manipularea microorganismelor sunt discutate in detaliu cu Conf. habil. dr. Marius Stefan, Universitatea ''Alexandru I Cuza'', Facultatea de Biologie.


Conferenţiar dr. Marius Stefan in laborator, Decembrie 2010, Iasi

  • Problemele legate de redactarea si trimiterea a manuscriselor, receptori neuronali si neurotransmitatori sunt rezolvate impreuna cu conf. dr. Lucian Hritcu
 

Last  man standing - Conferenţiar dr. Lucian Hritcu este ultimul care pleaca de la  EMBO Meeting 2011, Vienna, Austria

  • Multitudinea de probleme adminsitrative, precum si cele legate de chimie sunt dicutate si rezolvate cu ajutorul conf. dr. Zenovia Olteanu


Studenti straini

Jaurès AK Noumedem, doctorand, Departamentul de Biochimie, Facultatea de Stiinte, Universitatea din Dschang, Cameroon, Bursier ''Eugen Ionescu'',  AUF, 2011 Julie Reghem, masterand, DUT “Genie Biologique”, ABB – Lille, bursier Erasmus Placements, 2013 Victor Baumont, Student, DUT “Genie Biologique”, ABB – Lille, bursier Erasmus Placements, 2015




Fakhri Kallabi, PostDoc, Universitatea din  SFAX, Tunisia, Bursă AUF ''Eugen Ionescu'', 2020
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Legaturi

14/09/2012

Organizatii ce asigura finantarea cercetarii in Romania:

  • Autoritatea Nationala pentru Cercetare Stiintifica: ANCS

  • Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii: UEFISCDI


Soft pentru andocare moleculara si modelarea structurii 3D a proteinelor:

  • mediu complet, flexibil si modular de modelare moleculara si vizualizare - OpenStructure

  • server complet automat pentru modelarea structurii 3D pe baza de omologie  - SWISS-MODEL

  • o modalitate alternativa de a accesa si interactiona cu SWISS-MODEL - DeepView (Swiss Pdb-Viewer)

  • program pentru modelarea structii 3D a proteinelor pe baza de omologie sau modelare comparativa, nu are interfata grafica - MODELLER

  • o interfata grafica pentru Modeller - MINT

  • program pentru vizualizarea, editarea si analiza structurilor moleculelor - UCSF Chimera

  • suita de programe pentru protein engineering, in special pentru mutageneza (site-directed mutagenesis) -  TRITON

  • program de andocare moleculara, in linie de comanda - DOCK 6

  • o suita de instrumente pentru andocare moleculara automatizata  - AutoDock si o interfata grafica gratuita - ADT

  • unul dintre cele mai populare programe de vizualizare a moleculelor - PyMOL 

  • sistem de operare bootabil si autoconfigurabil pentru screening virtual bazat pe AutoDock4/Vina - MOLA

Software bioinformatic si Linux:

  • utilitare pentru genetică microbiană comparativă  Genomics BioTools  - CMGBiotools

  • briceagul elvetian al staiilor de lucru in bioinformatica - Bio-linux

  • proiect dedicat pentru dezvoltarea sistemului de operare Debian in scopul utilzarii in practica medicala si cercetare  Debian Med

  • o colectie de aplicatii open-source pentru cercetatorii din domeniul stiintelor vietii  BioBrew

  • un comentariu interesant despre Open software for biologists

Baze de date si alte legaturi utile:

  • colectie de metode diponibila on-line: Protocol on-line

  • European Bioinformatics Institute: EMBL-EBI

  • Medline at NIH: PubMed

  • Central European Science Publishers: Versita

  • SIB PROTEOMICS: Expasy

  • World Wide Protein Structure Database: wwPDB and its european partner PDBe

  • DNA and protein Sequnce Manipulation Suite accesibil pe site-ul original SMS sau pe acest site SMS

Companii de distributie reactivi si echipamente:

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